Difference between revisions of "Part:BBa K658009:Design"

 
(→‎Source)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K658009 short</partinfo>
 
<partinfo>BBa_K658009 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
BBa_K658001
+
BBa_K658003
  
 
===References===
 
===References===

Revision as of 03:37, 6 October 2011

a bacteria population-control device with lux pR-3 driven by lacl+pL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2347


Design Notes

This device is based on BBa_K658001. The mutated promoter lux pR-3 in the killer protein producer regulate the expression of killer protein LacZalpha-ccdB. The strength of promoters lux pR and its 3 mutants lux pR-3, lux pR-5, lux pR-3/5 were tested before they were used as regulators in a circuit. The strength of the lux pR-3 is related to the expression of the killer protein LacZalpha-ccdB. The higher strength a lux pR has, the lower cell density may be got in the steady state.


Source

BBa_K658003

References