Difference between revisions of "Part:BBa K567002"
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The tRNA(Arg) is under the control of ''sulA'' promoter induced by UV. tRNA expression is induced by 20-second UV exposure (the distance between the 20W lamp and the culture is 35cm) when the OD600 of the culture reaches 0.3. | The tRNA(Arg) is under the control of ''sulA'' promoter induced by UV. tRNA expression is induced by 20-second UV exposure (the distance between the 20W lamp and the culture is 35cm) when the OD600 of the culture reaches 0.3. | ||
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| + | [[image:11SJTUResult4AGGUV.jpg|frame|center]] | ||
| + | [[image:11SJTUResult6AGGUV.jpg|frame|center|''Fig''. the picture shows the change of luciferase activity before and after UV induction. As it is shown in the figure, reporter gene expression increased, indicating that tRNA is expressed after induction]] | ||
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Latest revision as of 03:34, 6 October 2011
sulA promoter-tRNA(Arg)
The tRNA(Arg) is under the control of sulA promoter induced by UV. tRNA expression is induced by 20-second UV exposure (the distance between the 20W lamp and the culture is 35cm) when the OD600 of the culture reaches 0.3.
Fig. the picture shows the change of luciferase activity before and after UV induction. As it is shown in the figure, reporter gene expression increased, indicating that tRNA is expressed after induction
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 177
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 177
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 177
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 177
- 1000COMPATIBLE WITH RFC[1000]