Difference between revisions of "Part:BBa K658001:Design"
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Revision as of 03:32, 6 October 2011
a bacteria population-control device with RBS1.0 driven by lacl+pL
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2344
Illegal BsaI.rc site found at 1874
Design Notes
The bacteria population-control system was once a two-plasmid system. In order to enhance its stability and efficiency, we integrated target sequences into one plasmid.
Source
K176003
K658003
References
[1] Kampranis SC, Howells AJ, Maxwell A. The interaction of DNA gyrase with the bacterialtoxin CcdB: evidence for the existence of two gyrase-CcdB complexes[J]. Journal of Molecular Biology, 1999, 293(3): 733-744.
[2] Dwyer DJ, Kohanski MA, Hayete B, Collins JJ. Gyrase inhibitors induce an oxidative damage cellular death pathway in Escherichia coli[J]. Molecular Systems Biology, 2007, 3(1): 91. Galhardo RS, Do R, Yamada M, Friedberg EC, Hastings PJ, Nohmi T, Rosenberg SM. DinB upregulation is the sole role of the SOS response in stress-induced mutagenesis in Escherichia coli[J]. Genetics, 2009, 182(1): 55-68.