Difference between revisions of "Part:BBa K081014:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | ===Part BBa_K572001 - RFP Generator with PcstA Carbon Stress Promoter=== | ||
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| + | <img src="https://static.igem.org/mediawiki/2011/e/ee/RFP_Generator_Experience_01.jpg" width="750px" height="971px" /> | ||
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| + | <img src="https://static.igem.org/mediawiki/2011/b/b9/PcstA_and_RFP_Generator_01.jpg" width="750px" height="971px" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/0/0e/PcstA_and_RFP_Generator_02.jpg" width="750px" height="971px" /> | ||
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===Applications of BBa_K081014=== | ===Applications of BBa_K081014=== | ||
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===User Reviews=== | ===User Reviews=== | ||
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Revision as of 03:30, 6 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Part BBa_K572001 - RFP Generator with PcstA Carbon Stress Promoter
Applications of BBa_K081014
Experiment 1 - performed by Lorenzo Pasotti and Mattia Quattrocelli
This generator was contained into pSB1AK3. The constitutive promoter BBa_J23100 was assembled upstream of the generator using BioBrick Standard Assembly.
After ligation reaction we transformed 1 ul of ligation product into 50 ul of Invitrogen TOP10 and plated transformed bacteria on LB agar plates with Ampicilline.
15 hours after plate incubation at 37°C, we put the plate on the transluminator. Positive transformants glowed under UV rays (see figure).
We also picked up some colonies from the plate using a tip and infected 1 ml of LB + Amp. After 3 hours of incubation (37°C, 220 rpm) we watched 50 ul of bacteria with TRITC channel. Results are reported in the following figure.
We can see that our RFP generator actually works.
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User Reviews
UNIQ951b6a6931a374a2-partinfo-00000001-QINU UNIQ951b6a6931a374a2-partinfo-00000002-QINU