Difference between revisions of "Part:BBa K567012"
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tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from ''aspV'' in E.coli, then the anticodon region is site-directed mutated. | tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from ''aspV'' in E.coli, then the anticodon region is site-directed mutated. | ||
− | ''Get more information from Biobrick'' | + | '''Get more information from Biobrick''' |
PT7-TDRS ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567011 BBa_K567011]) | PT7-TDRS ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567011 BBa_K567011]) |
Revision as of 03:18, 6 October 2011
tRNA(Asp)-AGG
tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter. This biobrick is constructed first by cloning the tRNA(Asp) from aspV in E.coli, then the anticodon region is site-directed mutated.
Get more information from Biobrick
PT7-TDRS (BBa_K567011)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 281
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 281
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 281
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 281
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 281
- 1000COMPATIBLE WITH RFC[1000]