Difference between revisions of "Part:BBa K572004"
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<partinfo>BBa_K572004 parameters</partinfo>
 
<partinfo>BBa_K572004 parameters</partinfo>
 
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The biobrick in pSB1A2 was transformed into DH5alpha cells. This primary culture was used as an inoculum for the secondary culture. After the OD reached 0.6, the cells were resuspended in M9 Media with different glucose concentrations - 0, 0.02, 0.1, 0.5, LB and LB+0.2.
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The biobrick contains a eukaryotic ribosome site. This experiment serves as a characterization of the ribosome binding site. The activity of luciferase was found to be close to the basal level even after 6 hours after introducing cells into M9 media. The eukaryotic ribosome binding site didn't result in expression of the protein following it in E.coli.
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Revision as of 03:05, 6 October 2011

PcstA_Luciferase Generator

The part is used as a control in our experiments with proteorhodopsin. RFP production is estimated using luciferase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 684


<! The biobrick in pSB1A2 was transformed into DH5alpha cells. This primary culture was used as an inoculum for the secondary culture. After the OD reached 0.6, the cells were resuspended in M9 Media with different glucose concentrations - 0, 0.02, 0.1, 0.5, LB and LB+0.2.

The biobrick contains a eukaryotic ribosome site. This experiment serves as a characterization of the ribosome binding site. The activity of luciferase was found to be close to the basal level even after 6 hours after introducing cells into M9 media. The eukaryotic ribosome binding site didn't result in expression of the protein following it in E.coli. >