|
|
| Line 1: |
Line 1: |
| | __NOTOC__ | | __NOTOC__ |
| − | =iGEM11_OUC Experience=
| |
| − |
| |
| − |
| |
| | ===User Reviews=== | | ===User Reviews=== |
| − | <!-- DON'T DELETE --><partinfo>BBa_K594015 StartReviews</partinfo>
| |
| − | {|width='80%' style='border:1px solid gray'
| |
| − | |-
| |
| − | |width='10%'|
| |
| − | <partinfo>BBa_K594015 AddReview 5</partinfo>
| |
| − | <I>DonQuixia</I>
| |
| − | |width='60%' valign='top'|
| |
| − | When we use the pSB1C3 for standardization, we found that the pSB1C3 have the right antibiotic resistance but a wrong DNA length.
| |
| − | |}
| |
| − | <!-- DON'T DELETE --><partinfo>BBa_K594015 EndReviews</partinfo>
| |
| − | ===The verification===
| |
| − | [[Image:OUC-China.Review.jpg|center]]
| |
| − | The pSB1C3 is supposed to be 2049bp, however, it appears around the marker 3000bp.
| |
| − | We have the pSB1C3 in K381001 sequenced and found its total length is 2944bp, and its total sequence is as below,
| |
| − | [[Image:OUC-China.ExpDNA.png|center]]
| |
| − | Through sequence analysis, we could find that the base in blue have a 99% homology with the right pSB1C3. It lacks a C and a A in the beginning. In the end, it has a 71 base homology with the right pSB1C3. The other sequence left do not accord with the right pSB1C3.
| |
