Difference between revisions of "Part:BBa K658016:Design"
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XXXXXXXXXXXXXXXXXXXXX Fig.1 XXXXXXXXXXXXXXXXXX | XXXXXXXXXXXXXXXXXXXXX Fig.1 XXXXXXXXXXXXXXXXXX | ||
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XXXXXXXXXXXXXXXXXXXXX Fig.2 XXXXXXXXXXXXXXXXXX | XXXXXXXXXXXXXXXXXXXXX Fig.2 XXXXXXXXXXXXXXXXXX | ||
Revision as of 02:45, 6 October 2011
LuxI->LuxR->lux pR->GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1874
Illegal BsaI.rc site found at 2601
Design Notes
Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device.
The device is constructed by combining two parts.
XXXXXXXXXXXXXXXXXXXXX Fig.1 XXXXXXXXXXXXXXXXXX
The two parts are proven to be right.
XXXXXXXXXXXXXXXXXXXXX Fig.2 XXXXXXXXXXXXXXXXXX
From the electrophoretogram,we can see that the part is successfully constructed.
Source
K658012