Difference between revisions of "Part:BBa K658016:Design"
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<partinfo>BBa_K658016 short</partinfo> | <partinfo>BBa_K658016 short</partinfo> | ||
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Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device. | Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device. | ||
| + | The device is constructed by combining two parts. | ||
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| + | XXXXXXXXXXXXXXXXXXXXX Fig.1 XXXXXXXXXXXXXXXXXX | ||
| + | |||
| + | The two parts are proven to be right. So we constructed the new parts by combining them. | ||
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| + | XXXXXXXXXXXXXXXXXXXXX Fig.2 XXXXXXXXXXXXXXXXXX | ||
| + | From the electrophoretogram,we can see that the part is successfully constructed. | ||
===Source=== | ===Source=== | ||
Revision as of 02:44, 6 October 2011
LuxI->LuxR->lux pR->GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1874
Illegal BsaI.rc site found at 2601
Design Notes
Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device.
The device is constructed by combining two parts.
XXXXXXXXXXXXXXXXXXXXX Fig.1 XXXXXXXXXXXXXXXXXX
The two parts are proven to be right. So we constructed the new parts by combining them.
XXXXXXXXXXXXXXXXXXXXX Fig.2 XXXXXXXXXXXXXXXXXX
From the electrophoretogram,we can see that the part is successfully constructed.
Source
K658012