Difference between revisions of "Part:BBa K629003"
(→Picture of Chemotaxis in cheZ knocked-out strain) |
|||
| Line 22: | Line 22: | ||
== Picture of Chemotaxis in ''cheZ'' knocked-out strain == | == Picture of Chemotaxis in ''cheZ'' knocked-out strain == | ||
| + | The main purpose of cheZ is to control chemotaxis in E. Coli. What we have done is to use different promoter to test how to get the better result from expression of CheZ. Below are pictures and parameters of chemotaxis test. | ||
Revision as of 02:30, 6 October 2011
cheZ, chemotaxis regulator, protein phosphatase for CheY
Background
Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
Expression in E. Coli
Before we started our functional test of CheZ, we have utilized western blot to determine whether there are enough soluble proteins in E. Coli. With 6×His tag on pET-32a, the result shows that CheZ has more expression with induction of IPTG.
Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some cheZ without induction of IPTG.
Picture of Chemotaxis in cheZ knocked-out strain
The main purpose of cheZ is to control chemotaxis in E. Coli. What we have done is to use different promoter to test how to get the better result from expression of CheZ. Below are pictures and parameters of chemotaxis test.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]