Difference between revisions of "Part:BBa K594015:Experience"

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===User Reviews===
 
===User Reviews===
 
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<I>DonQuixia</I>
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<I>EliasGrimaldo</I>
 
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When we use the pSB1C3 for standardization, we found that the pSB1C3 have the right antibiotic resistance but a wrong DNA length. This can be clearly seen from the electrophoretogram below.
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The part work well under cold shock stress (20°C to 30°C) of 10-12 hr.
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Revision as of 02:04, 6 October 2011

Applications of BBa_K594015

From the beginning, we use the linearized plasmid backbone pSB1C3 in the 2011 spring DNA distribution. But the standardization with them all end up with failures.

Then, we began to seek for a new plasmid backbone. We found the K381001 in pSB1C3. If we cut the pSB1C3 with EcoR I and Pst I, we could get pSB1C3 plasmid backbone for standardization.

User Reviews

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