Difference between revisions of "Part:BBa K567013"
Line 2: | Line 2: | ||
<partinfo>BBa_K567013 short</partinfo> | <partinfo>BBa_K567013 short</partinfo> | ||
− | tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of | + | tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated. |
Revision as of 00:59, 6 October 2011
Status: 500 Content-type: text/html
Software error:
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.
Construction of BBa_K567013
This biobrick is constructed first by cloning the tRNAAsp from AspV in E.coli, then the anticodon region is site-directed mutated. This part is constructed on the backbone plasmid pACYC184.
Characterization of BBa_K567013
This part acts as a stop codon suppressor tRNA. It can be charged with Asp.
We have used Pbla-Luc-TAG as our Reporter. The amount of luciferase produced is reflected using the bioluminescence emitted during the luciferin reaction. Our results demonstrate that this part can suppress TAG insertion into luciferase. In the experimental group, with the help of BBa_K567011 PT7-TDRS (Favorite Part), luciferase-TAG was produced and bioluminescence was emitted during the luciferin reaction. These results proved that this tRNAAsp can effectively suppress stop codon.
![](/wiki/images/2/2b/11SJTU-stop-codon_switch.jpg)
Sequence and Features Status: 500 Content-type: text/html
Software error:
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.