Difference between revisions of "Part:BBa K567012"

Revision as of 00:56, 6 October 2011

tRNA(Asp)-AGG

tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter. This biobrick is constructed first by cloning the tRNA(Asp) from aspV in E.coli, then the anticodon region is site-directed mutated.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 281
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 281
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 281
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 281
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 281
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K567012 short</partinfo>
-tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.
+tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from ''aspV'' in E.coli, then the anticodon region is site-directed mutated.
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