Difference between revisions of "Part:BBa K594004:Design"

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===Source===
 
===Source===
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We got the S. meliloti 1021 from Nanjing Agriculture University, and then cloned the sinI gene from S. meliloti 1021 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.
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We got the Sinorhizobium meliloti Rm 1021 from Nanjing Agriculture University, but we fail to get the sequence of psinI, so we deduct a 500bp sequence that may cover the promoter. then cloned the deducted psinI gene from Sinorhizobium meliloti Rm 1021 with primers designed by the part design tool in the website [ginkgobioworks.com].
  
 
===References===
 
===References===

Latest revision as of 00:42, 6 October 2011

the promoter of sinI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
    Illegal NgoMIV site found at 251
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in S. meliloti Rm 1021, and then found the specific sequence in NCBI and asked the S. meliloti Rm 1021 strain from Nanjing Agricultural University. The promoter of sinI have no threats to biosafety.

Source

We got the Sinorhizobium meliloti Rm 1021 from Nanjing Agriculture University, but we fail to get the sequence of psinI, so we deduct a 500bp sequence that may cover the promoter. then cloned the deducted psinI gene from Sinorhizobium meliloti Rm 1021 with primers designed by the part design tool in the website [ginkgobioworks.com].

References

1.Melanie M. Marketon and Juan E. González. 2002.Identification of Two Quorum-Sensing Systems in Sinorhizobium meliloti. JOURNAL OF BACTERIOLOGY, p. 3466–3475July 2002
2.Melanie M. Marketon, Matthew R. Gronquist,2 Anatol Eberhard,3 and Juan E. Gonza´lez1. 2002,Characterization of the Sinorhizobium meliloti sinR/sinI Locus and the Production of Novel N-Acyl Homoserine Lactones,JOURNAL OF BACTERIOLOGY, p. 5686–5695
3.Juan E. Gonza´lez and Melanie M. Marketon. 2003, Quorum Sensing in Nitrogen-Fixing Rhizobia, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, p. 574–592