Difference between revisions of "Part:BBa K594008:Design"
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| − | + | We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiI gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers. | |
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===References=== | ===References=== | ||
Revision as of 00:25, 6 October 2011
a luxR-type regulator from Rhizobium etli CFN42
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 248
Illegal NgoMIV site found at 344
Illegal NgoMIV site found at 508 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.
Source
We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiI gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.