Difference between revisions of "Part:BBa K594007:Design"
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===Source=== | ===Source=== | ||
| − | + | We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiI gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers. | |
===References=== | ===References=== | ||
Revision as of 00:16, 6 October 2011
a luxI homologous AHL synthetase from Rhzobium etli CFN 42
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 28
Illegal XhoI site found at 583 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 403
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 31
Design Notes
The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.
Source
We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiI gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.