Difference between revisions of "Part:BBa K594005:Design"

 
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===Design Notes===
 
===Design Notes===
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Initial experiments showed 3-OH-C14:1-HSL to be an inducer of the rhi genes, but later data showing that rhiI and rhiA could be induced by short-chain AHLs (C6-HSL, C8-HSL, and 3-oxo-C8-HSL) but not 3-OH-C14:1-HSL suggested that the induction was indirect.
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The rhi quorum sensing system show relationships with plasmid transfering. However, real transferring only occurs when these genes are combined with other essential genes like traABC, traI/R, so only rhi quorum sensing could not make the plasmid transfer. They will not arouse biosafety problems.
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===Source===
 
===Source===

Revision as of 23:12, 5 October 2011

a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rhi quorum sensing system show relationships with plasmid transfering. However, real transferring only occurs when these genes are combined with other essential genes like traABC, traI/R, so only rhi quorum sensing could not make the plasmid transfer. They will not arouse biosafety problems.

Source

it is from the Rhzobium leguminosarum bv. vicae IMA57 we purchased from the CGMCC(China General Microbiological Culture Collection Center).

References