Difference between revisions of "Part:BBa K624053"

Revision as of 22:50, 5 October 2011

mcherry+minC+RBS+tetO+pT7 + pT7+RBS+tetR+GFP

mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

This part is composed of BBa_K624041 and BBa_K624002, both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order). We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 803
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 803
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 803
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 803
Illegal AgeI site found at 681
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2898

@@ Line 4: / Line 4: @@
 mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP
-This part is composed of BBa_K624041 and BBa_K624002(the former subpart is in reverse order). We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli.
+This part is composed of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624041 BBa_K624041] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624002 BBa_K624002], both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order). We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli.
 <!-- Add more about the biology of this part here