Difference between revisions of "Part:BBa K624053"
Line 3: Line 3:
  
 
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP
 
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP
 +
 +
This part is composed of BBa_K624041 and BBa_K624002(the former subpart is in reverse order). We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
−
This part is composed of BBa_K624041 and BBa_K624002(the former subpart is in reverse order). We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli.
 
  
 
<!-- -->
 
<!-- -->

Revision as of 22:47, 5 October 2011

mcherry+minC+RBS+tetO+pT7 + pT7+RBS+tetR+GFP

mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

This part is composed of BBa_K624041 and BBa_K624002(the former subpart is in reverse order). We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 803
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 803
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 803
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 803
    Illegal AgeI site found at 681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2898