Difference between revisions of "Part:BBa K624033"

Revision as of 22:23, 5 October 2011

minC cell division inhibitor (revised)

This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team(BBa_J29040), but there is not an available part. 2010 Warsaw iGEM team submitted another minC (BBa_K299806) with different sequence. However, there was a PstI site in the middle of the sequence.

We have cloned the minC gene from E. coli K-12 strain MG1655 by performing PCR, with a single nucleotide synonymous mutation to eliminate the PstI site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 211
1000
COMPATIBLE WITH RFC[1000]

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 This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team([https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J29040 BBa_J29040]), but there is not an available part. 2010 Warsaw iGEM team submitted another minC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K299806 BBa_K299806]) with different sequence. However, there was a PstI site in the middle of the sequence.
-We designed primers to remove PstI the site with a synonymous substitution. This makes it easier to do EXSP biobrick assembly.
+We have cloned the ''minC'' gene from E. coli K-12 strain MG1655 by performing PCR, with a single nucleotide synonymous mutation to eliminate the PstI site.
 <!-- Add more about the biology of this part here