Difference between revisions of "Part:BBa K624001"

Latest revision as of 22:03, 5 October 2011

tetR+RBS+GFP+ter

This part is composed of tetR and gfp.

These are the measurements of our construct tetR+RBS+GFP under fluorescence microscope. The left graph is from the control group of E. coli strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in E. coli, while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.

GFP measurements under different excitation wavelengths

The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm. In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition. Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1358

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-[[Image:30.JPG]][[Image:31.JPG]][[Image:32.JPG]]
+{| class="wikitable" border="0"
+|-
+!  [[Image:30.JPG|250px|]]
+!  [[Image:31.JPG|250px|]]
+!  [[Image:32.JPG|250px|]]
+|-
+|}
-'''These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left, above graph is control group of E.coli strain DH5a. The right, above is tetR+RBS+GFP in plasmid c3 in E.coli , while the below is plate without any bacilli. All graphs are measured in fixed exposure time.   Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing work is under operation.'''
+<font size=3>These are the measurements of our construct tetR+RBS+GFP under fluorescence microscope. The left graph is from the control group of ''E. coli'' strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in ''E. coli'', while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.
+'''GFP measurements under different excitation wavelengths'''
+[[Image:33.JPG]]
+<font size=3>The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm.  In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition.  Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.