Difference between revisions of "Part:BBa K624001"
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<partinfo>BBa_K624001 short</partinfo>
 
<partinfo>BBa_K624001 short</partinfo>
  
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Add a promoter and a RBS on the upstream will express tetR protein and GFP.
+
This part is composed of ''tetR'' and ''gfp''.
  
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GFP can help detecting tetR expression.
 
  
 
[[Image:30.JPG]][[Image:31.JPG]][[Image:32.JPG]]
 
[[Image:30.JPG]][[Image:31.JPG]][[Image:32.JPG]]

Revision as of 21:56, 5 October 2011

tetR+RBS+GFP+ter

This part is composed of tetR and gfp.


30.JPG31.JPG32.JPG

These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left, above graph is control group of E.coli strain DH5a. The right, above is tetR+RBS+GFP in plasmid c3 in E.coli , while the below is plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing work is under operation.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1358