Difference between revisions of "Part:BBa K581004"
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<h3>Methods<a href="http://2011.igem.org/Team:Peking_S/lab/protocol"><img src="https://static.igem.org/mediawiki/igem.org/d/d9/PkusSubtitleshort.png" width="240px" align="right"/></a></h3>
 
<h3>Methods<a href="http://2011.igem.org/Team:Peking_S/lab/protocol"><img src="https://static.igem.org/mediawiki/igem.org/d/d9/PkusSubtitleshort.png" width="240px" align="right"/></a></h3>
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<h2>References</h2>
 
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<p>[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator.  <i>The EMBO journal </i> <b>23:</b> 396-405</p>
 
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<p>[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. <i>Molecular microbiology</i><b> 61:</b> 1013-1022</p>
 
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<p>[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA.  <i> PLoS biology </i><b>5: </b>e229
 
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<h2>References</h2>
 
<h2>References</h2>

Revision as of 21:48, 5 October 2011

ptsG2-GFP (ptsG2 5'UTR fused with gfp)

This is the conjugate part of the small RNA regulator sgrS2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747




This BioBrick has been sequence verified.

Background

SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).

miao

Experimental Data

Methods

References

[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022

[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229