Difference between revisions of "Part:BBa K557002:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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<p>&nbsp;&nbsp;&nbsp;&nbsp;The Aptamer-cheZ Device(without lac promoter) was subcloned into the SpeI and PstI sites of the Toggle Switch Device([https://parts.igem.org/Part:BBa_K228003 BBa_K228003]) from PKU to create the Toggle Switch -Aptamer-cheZ Device. After the transformation, diluted cell suspensions from mid-log-phase cultures were applied to the center of the Semi-Solid Media(as shown in Figure4.). The plates were dried the room temperature at for 15 min, and incubated at 37&deg;C for 10h, and the migration radii were determined by measuring the diameter of the outermost ring of growth.</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;The Aptamer-cheZ Device(without lac promoter) was subcloned into the SpeI and PstI sites of the Toggle Switch Device([https://parts.igem.org/Part:BBa_K228003 BBa_K228003]) from PKU to create the Toggle Switch -Aptamer-cheZ Device.
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[[File:D().jpg|center|350px| Figure4.]]
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<p align=center class="ppp">Figure4.</p>
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<html><a name="Toggle Switch-Aptamer-cheZ" id=""></a></html>
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===Source===
 
===Source===

Latest revision as of 21:43, 5 October 2011

Toggle-switch+Aptamer+CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

    The Aptamer-cheZ Device(without lac promoter) was subcloned into the SpeI and PstI sites of the Toggle Switch Device(BBa_K228003) from PKU to create the Toggle Switch -Aptamer-cheZ Device.

Source

The part is made up of the standard parts from parts.igem.

===References===