Difference between revisions of "Part:BBa K557002:Design"

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===Design Notes===
 
===Design Notes===
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<p>&nbsp;&nbsp;&nbsp;&nbsp;The Aptamer-cheZ Device(without lac promoter) was subcloned into the SpeI and PstI sites of the Toggle Switch Device([https://parts.igem.org/Part:BBa_K228003 BBa_K228003]) from PKU to create the Toggle Switch -Aptamer-cheZ Device. After the transformation, diluted cell suspensions from mid-log-phase cultures were applied to the center of the Semi-Solid Media(as shown in Figure4.). The plates were dried the room temperature at for 15 min, and incubated at 37&deg;C for 10h, and the migration radii were determined by measuring the diameter of the outermost ring of growth.</p>
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[[File:D().jpg|center|350px| Figure4.]]
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<p align=center class="ppp">Figure4.</p>
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<html><a name="Toggle Switch-Aptamer-cheZ" id=""></a></html>
  
 
===Source===
 
===Source===

Revision as of 21:41, 5 October 2011

Toggle-switch+Aptamer+CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

    The Aptamer-cheZ Device(without lac promoter) was subcloned into the SpeI and PstI sites of the Toggle Switch Device(BBa_K228003) from PKU to create the Toggle Switch -Aptamer-cheZ Device. After the transformation, diluted cell suspensions from mid-log-phase cultures were applied to the center of the Semi-Solid Media(as shown in Figure4.). The plates were dried the room temperature at for 15 min, and incubated at 37°C for 10h, and the migration radii were determined by measuring the diameter of the outermost ring of growth.

Figure4.

Figure4.

Source

The part is made up of the standard parts from parts.igem.

References