Difference between revisions of "Part:BBa K598008"

Latest revision as of 20:02, 5 October 2011

TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015

This is a basic part that consists of thiamine pyrophosphate (TPP) ligand responsive hammerhead ribozyme numbered 2.5 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.(fig.1)

Figure 1 Construction of TPP UP-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015. This part consists of TPP ribozyme 1.20 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.It is obtained by PCR from TPP-HHAz 1.20 [1] which is kindly provided by Markus Wieland et al., and then inserted into pSB1C3 through standard assembly.

This part is obtained by PCR from TPP-HHAZ 1.20 [1] which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.

Reference

[1] Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756

Sequence and Features