Difference between revisions of "Part:BBa K544001"

Revision as of 18:41, 5 October 2011

Tetracycline Repressor with Consitutive Promoter BBa_J23103

A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 65
Illegal SpeI site found at 37
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 65
Illegal SpeI site found at 37
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 65
Illegal SpeI site found at 37
1000
COMPATIBLE WITH RFC[1000]

Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors (The data collected are considered only when difference between values with the P-value <0.05)

Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors (The data collected are considered only when difference between values with the P-value <0.05) [EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]

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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K544001 SequenceAndFeatures</partinfo>
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