Difference between revisions of "Part:BBa K581022:Design"
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Revision as of 18:38, 5 October 2011
A-factor regulated promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 12
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We created the arpA-binding promoter by positioning the A-factor receptor protein(ArpA) binding sequence between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When A-factor represents and binds to ArpA proteins, the protein structure of ArpA changes and ArpA has less affinity to its DNA binding site, thus to increase the accessibility of RNA polymerases to this promoter, activating the transcription initiation.
Source
We build this promoter from the ground up