Difference between revisions of "Part:BBa K581022:Design"

 
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===Design Notes===
 
===Design Notes===
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Insertion of the A-factor receptor protein(ArpA) binding sequence in a constitutive promoter.
 
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We created the arpA-binding promoter by positioning the A-factor receptor protein(ArpA) binding sequence between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When A-factor represents and binds to ArpA proteins, the protein structure of ArpA changes and ArpA has less affinity to its DNA binding site, thus to increase the accessibility of RNA polymerases to this promoter, activating the transcription initiation.
  
 
===Source===
 
===Source===

Revision as of 18:38, 5 October 2011

A-factor regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We created the arpA-binding promoter by positioning the A-factor receptor protein(ArpA) binding sequence between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When A-factor represents and binds to ArpA proteins, the protein structure of ArpA changes and ArpA has less affinity to its DNA binding site, thus to increase the accessibility of RNA polymerases to this promoter, activating the transcription initiation.

Source

Synthesized.

References