Difference between revisions of "Part:BBa K539281"
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This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. crtZ is regulated by cI and cI regulated promoter. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. | This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. crtZ is regulated by cI and cI regulated promoter. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. | ||
+ | [[Image:pathway]] | ||
+ | [[Image:crtZ]] | ||
+ | When we increase the temperature to 42℃, the CI proteins BBa_K098997, the repressor of cI regulated promoter BBa_R0051, are fully denatured. Then the cI regulated promoter is activated and CrtZ BBa_I742158 is produced, converting the orange beta-carotene to yellow Zeaxanthin. | ||
− | + | This biobrick can coorperate with [https://parts.igem.org/Part:BBa_K539151 BBa_K539151] | |
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Revision as of 17:57, 5 October 2011
rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI regulater
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. crtZ is regulated by cI and cI regulated promoter. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. File:Pathway File:CrtZ When we increase the temperature to 42℃, the CI proteins BBa_K098997, the repressor of cI regulated promoter BBa_R0051, are fully denatured. Then the cI regulated promoter is activated and CrtZ BBa_I742158 is produced, converting the orange beta-carotene to yellow Zeaxanthin.
This biobrick can coorperate with BBa_K539151
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1492
- 1000COMPATIBLE WITH RFC[1000]