Difference between revisions of "Part:BBa K533006:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K533006===
 
===Applications of BBa_K533006===
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This is the expression device of mCherry.
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When IPTG is added, strong expression of mCherry can be detected. However, mCherry cannot be properly folded above 20 centigrade, as shown by our experimental results.
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{| align="center" style="border: none; background: none; border-spacing: 0;text-align:center;margin-left: 100px;"
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! style="border: 1px solid #39F;padding: 5px;" | Group
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| style="border: 1px solid #39F;padding: 5px;" | 1
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| style="border: 1px solid #39F;padding: 5px;" | 2
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|-
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| style="border: 1px solid #39F;padding: 5px;" |  Volume
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| style="border: 1px solid #39F;padding: 5px;" colspan="2" | 3ml
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|-
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| style="border: 1px solid #39F;padding: 5px;"  rowspan="3" | IPTG(mM)
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| style="border: 1px solid #39F;padding: 5px;"  colspan="2" | 0.1
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|-
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| style="border: 1px solid #39F;padding: 5px;"  colspan="2" | 0.5
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|-
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| style="border: 1px solid #39F;padding: 5px;"  colspan="2" | 1.0
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|-
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| style="border: 1px solid #39F;padding: 5px;" | Temperature
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| style="border: 1px solid #39F;padding: 5px;" |  30<html><sup>o</sup>C</html>
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| style="border: 1px solid #39F;padding: 5px;" |  18<html><sup>o</sup>C</html>
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|-
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| style="border: 1px solid #39F;padding: 5px;" |  Duration
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| style="border: 1px solid #39F;padding: 5px;" |  12h
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| style="border: 1px solid #39F;padding: 5px;" |  12h
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|-
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| style="border: 1px solid #39F;padding: 5px;" |  Result
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| style="border: 1px solid #39F;padding: 5px;" |  Media remained yellowish.<html><br /></html> E. coli white
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|  style="border: 1px solid #39F;padding: 5px;" | Media turned purple. <html><br /></html>E. coli cherry pink
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|}
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After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression)
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[[File:Thuexp_cherryexp.png]]
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''From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.''
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:11, 5 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K533006

This is the expression device of mCherry.

When IPTG is added, strong expression of mCherry can be detected. However, mCherry cannot be properly folded above 20 centigrade, as shown by our experimental results.

Group 1 2
Volume 3ml
IPTG(mM) 0.1
0.5
1.0
Temperature 30oC 18oC
Duration 12h 12h
Result Media remained yellowish.
E. coli white 		Media turned purple.
E. coli cherry pink

After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression)

Thuexp cherryexp.png

From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.

User Reviews

UNIQ24d913458349038f-partinfo-00000004-QINU UNIQ24d913458349038f-partinfo-00000005-QINU