Difference between revisions of "Part:BBa K533009"

Latest revision as of 16:03, 5 October 2011

Tar receptor weak expression cassette

Tar receptor is now under the drive of lacI promoter, a constitutive weak promoter. In addition, the Shine-Dalgarno sequence is removed to lower translation efficiency. Lower expression level ensures more specific chemotaxis and better engineering of movement control.

Usage and Biology

pPD10, the empty vector demonstrated no significant chemotaxis while pPD12 vector demonstrated strong chemotaxis. We thus cloned the expression cassette from pPD12 and use it for our chemotaxis experiments.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1074
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1074
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1074
Illegal AgeI site found at 1936
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2514
Illegal BsaI site found at 3582
Illegal SapI.rc site found at 765

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K533009 short</partinfo>
@@ Line 5: / Line 4: @@
 Tar receptor is now under the drive of lacI promoter, a constitutive weak promoter. In addition, the Shine-Dalgarno sequence is removed to lower translation efficiency. Lower expression level ensures more specific chemotaxis and better engineering of movement control.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[Image:mgoulian_tar.jpg]]
+pPD10, the empty vector demonstrated no significant chemotaxis while pPD12 vector demonstrated strong chemotaxis. We thus cloned the expression cassette from pPD12 and use it for our chemotaxis experiments.
 <!-- -->