Difference between revisions of "Part:BBa K581010"
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Regulatory proteins for receiving signaling of CAI-1. | Regulatory proteins for receiving signaling of CAI-1. | ||
Revision as of 16:03, 5 October 2011
promoter(BBa_J23106)-RBS-luxU-RBS-cqsS-RBS-luxO
Regulatory proteins for receiving signaling of CAI-1.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 4021
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 4021 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3096
Illegal BamHI site found at 404 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 4021
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 4021
Illegal AgeI site found at 2374 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 964
Illegal SapI site found at 3284
Design Notes
▪ A silent mutation (A->G) at base 1086 was made to remove PstI site in the region of cqsS gene
▪ A silent mutation (A->G) at base 1650 was made to remove EcoRI site in the region of cqsS gene
▪ A silent mutation (G->A) at base 3234 was made to remove PstI site in the region of luxU gene
▪ cqsS and luxO were amplified by PCR
▪ promoter Bba_J23106 + luxU was amplified by DNAWorks
Source
▪ We received the genome DNA of Vibrio cholera from Chinese Center for Disease Control and Prevention
▪ Although our system includes several genes from Vibrio cholera, these genes are not directly involved with virulence or antibiotic resistance in any case.
References
- [http://nar.oxfordjournals.org/content/30/10/e43.full.pdf+html] David M. Hoover, Jacek Lubkowski. DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. Nucl. Acids Res 30 (10): e4 (2002).