Difference between revisions of "Part:BBa K543015"
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'''Repress expression of GFP by Anti-killing device'''<br> | '''Repress expression of GFP by Anti-killing device'''<br> | ||
| − | [[Image:Tokyo_Metropolitan_Killing2.jpg|right|]] | + | [[Image:Tokyo_Metropolitan_Killing2.jpg|right|250px]] |
We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. <br> | We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. <br> | ||
Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. <br> | Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. <br> | ||
Revision as of 15:51, 5 October 2011
TetR generator (J23100-B0031-R0040-B0015)
TetR generator (J23100-B0031-R0040-B0015).
Repress expression of GFP by Anti-killing device
We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence.
Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control.
Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working.
Need to more experience to get quantitative date.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]