Difference between revisions of "Part:BBa K543015"

Revision as of 15:38, 5 October 2011

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).

Repress expression of GFP by Anti-killing device
We confirm whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observe its florescence.
Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working.
Need to more experience to get quantitative date.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 TetR generator (J23100-B0031-R0040-B0015).
-'''Repress expression of GFP by Anti-killing device'''
+'''Repress expression of GFP by Anti-killing device'''<br>
 We confirm whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observe its florescence. <br>
 Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working. <br>
-For perfectly repressed expression cording under Ptet, RBS is needed to be more strong(ex.B0034).In addition, we should do more experience to get quantitative date.
+Need to more experience to get quantitative date.
 </td><img src="https://static.igem.org/mediawiki/2011/c/cd/Tokyo_Metropolitan_Killing2.png" width="300"><br><div align="right">