Difference between revisions of "Part:BBa K533005:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K533005===
 
===Applications of BBa_K533005===
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This is a surface marker for E. coli, although it can serve other purposes.
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We verified its membrane location by different means.
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In order to determine whether GFP is presented outside the membrane, cells were digested with Protease K at 50 centigrade for 30min.
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{| align="center" style="border: none; background: none; border-spacing: 0;text-align:center;margin-left: 100px;"
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! style="border: 1px solid #39F;padding: 5px;" |  Color Channel
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! style="border: 1px solid #39F;padding: 5px;" |  Post-digest
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! style="border: 1px solid #39F;padding: 5px;" |  Pre-digest
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|-
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|  style="border: 1px solid #39F;padding: 5px;" | GFP
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| style="border: 1px solid #39F;padding: 5px;" |  [[Image:Thuexp_gn.png|140px]]
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| style="border: 1px solid #39F;padding: 5px;" |  [[Image:Thuexp_gp.png|140px]]
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|-
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| style="border: 1px solid #39F;padding: 5px;" |  DAPI
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| style="border: 1px solid #39F;padding: 5px;" |  [[Image:Thuexp_gn_d.png|140px]]
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| style="border: 1px solid #39F;padding: 5px;" |  [[Image:Thuexp_gp_d.png|140px]]
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|-
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| style="border: 1px solid #39F;padding: 5px;" |  Merge
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| style="border: 1px solid #39F;padding: 5px;" |  [[Image:Thuexp_gn_m.png|140px]]
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| style="border: 1px solid #39F;padding: 5px;" |  [[Image:Thuexp_gp_m.png|140px]]
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|}
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The fluorescence disappeared after digesion, suggesting that the majority of GFP is on the outside of E. coli.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 15:35, 5 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K533005

This is a surface marker for E. coli, although it can serve other purposes.

We verified its membrane location by different means.


In order to determine whether GFP is presented outside the membrane, cells were digested with Protease K at 50 centigrade for 30min.

Color Channel Post-digest Pre-digest
GFP Thuexp gn.png Thuexp gp.png
DAPI Thuexp gn d.png Thuexp gp d.png
Merge Thuexp gn m.png Thuexp gp m.png

The fluorescence disappeared after digesion, suggesting that the majority of GFP is on the outside of E. coli.

User Reviews

UNIQ328076d9a51e66e0-partinfo-00000000-QINU UNIQ328076d9a51e66e0-partinfo-00000001-QINU