Difference between revisions of "Part:BBa K533002:Design"

 
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<partinfo>BBa_K533002 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The HIV protease site is embedded in the protein so that a proteolytic cleavage can release the binding cassette.
 
  
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Compared to part [[Part:BBa_K533001 | BBa_K533001]], we inserted an HIV-protease site between OmpA and SH3 to allow a proteolytic release of the cargo.
  
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This device is also under the drive of T7 promoter and lac repressor, allowing for strong inducible expression with IPTG in BL21 E. coli strain.
  
 
===Source===
 
===Source===

Latest revision as of 15:03, 5 October 2011

OmpA-HIV protease site-SH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 857
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compared to part BBa_K533001, we inserted an HIV-protease site between OmpA and SH3 to allow a proteolytic release of the cargo.

This device is also under the drive of T7 promoter and lac repressor, allowing for strong inducible expression with IPTG in BL21 E. coli strain.

Source

OmpA is from BL21(DE3) E. Coli genomic DNA. SH3 is from human Grb2 protein. HIV protease site is from a former part.

References