Difference between revisions of "Part:BBa K533003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K533003 short</partinfo>
 
<partinfo>BBa_K533003 short</partinfo>
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OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.
 
OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  
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We tested the function of this part. Our part [[Part:BBa_K533002 | BBa_K533002]] contained an HIV-protease site.
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After BBa_K533002 was bound to mCherry, we cleaved it with bacteria expressing this part.
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{| style="background: none; text-align:center;" align="center"
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| Color Channel
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| + E. coli expressing protease
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| + E. coli without protease
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|-
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| mCherry
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| [[Image:Thuexp_release_p.png|350px]]
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| [[Image:Thuexp_release_n.png|350px]]
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|-
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| DAPI
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| [[Image:Thuexp_release_p_d.png|350px]]
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| [[Image:Thuexp_release_n_d.png|350px]]
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|-
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| merge
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| [[Image:Thuexp_release_p_m.png|350px]]
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| [[Image:Thuexp_release_n_m.png|350px]]
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|}
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It shows that it works.
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We further optimized the conditions for expression induction.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K533003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K533003 SequenceAndFeatures</partinfo>

Revision as of 14:57, 5 October 2011

OmpA-HIV aspartyl protease

OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.


Usage and Biology

We tested the function of this part. Our part BBa_K533002 contained an HIV-protease site. After BBa_K533002 was bound to mCherry, we cleaved it with bacteria expressing this part.

Color Channel + E. coli expressing protease + E. coli without protease
mCherry Thuexp release p.png Thuexp release n.png
DAPI Thuexp release p d.png Thuexp release n d.png
merge Thuexp release p m.png Thuexp release n m.png

It shows that it works.

We further optimized the conditions for expression induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]