Difference between revisions of "Part:BBa I712667:Experience"
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Revision as of 14:33, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I712667
User Reviews
UNIQda24716643b491e2-partinfo-00000000-QINU
|
No review score entered. Yunxiao |
We fused this protease to the C-terminal of OmpA and tested its cutting efficiency with a membrane protein containing its cleavage site. We induced OmpA-protease fusion protein with IPTG concentration of 0.1mM, 0.5mM and 1mM. After 12hours induction at 18 centigrade, protease activity were observed in all the samples.
Protease cleavage will extinguish the red fluorescence from mCherry. As is shown, the cleavage activity is highest at 0.1mM induction, suggesting the too high expression level might hinder protein folding. |
UNIQda24716643b491e2-partinfo-00000002-QINU