Difference between revisions of "Part:BBa I712667:Experience"
(→User Reviews) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 18: | Line 17: | ||
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_I712667 AddReview number</partinfo> | ||
| + | <I>Yunxiao</I> | ||
| + | |width='60%' valign='top'| | ||
| + | We fused this protease to the C-terminal of OmpA and tested its cutting efficiency with a membrane protein containing its cleavage site. | ||
| + | |||
| + | We induced OmpA-protease fusion protein with IPTG concentration of 0.1mM, 0.5mM and 1mM. After 12hours induction at 18 centigrade, protease activity were observed in all the samples. | ||
| + | |||
| + | |||
| + | |}; | ||
<!-- DON'T DELETE --><partinfo>BBa_I712667 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_I712667 EndReviews</partinfo> | ||
Revision as of 14:28, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I712667
User Reviews
UNIQa820e2e4cb9365aa-partinfo-00000000-QINU
|
No review score entered. Yunxiao |
We fused this protease to the C-terminal of OmpA and tested its cutting efficiency with a membrane protein containing its cleavage site. We induced OmpA-protease fusion protein with IPTG concentration of 0.1mM, 0.5mM and 1mM. After 12hours induction at 18 centigrade, protease activity were observed in all the samples.
|
UNIQa820e2e4cb9365aa-partinfo-00000002-QINU