Difference between revisions of "Part:BBa K524001:Design"
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===Source=== | ===Source=== | ||
| − | Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908 | + | Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, [https://parts.igem.org/Part:BBa_I746908 BBa_I746908] |
===References=== | ===References=== | ||
Latest revision as of 14:24, 5 October 2011
pLac + RBS + split sfGFP 1-10
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 238
Design Notes
A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10. RBS was introduced into primers for cloning.
Source
Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908
References
Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107
Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854
Jun Zhou, Jian Lin, Cuihong Zhou, Xiaoyan Deng and Bin Xia.(2011).An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein.Acta Biochim Biophys Sin 43 (3): 239-244.