Difference between revisions of "Part:BBa K524007:Design"

(Source)
 
Line 10: Line 10:
 
===Source===
 
===Source===
  
Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908
+
Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, [https://parts.igem.org/Part:BBa_I746908 BBa_I746908]
  
 
===References===
 
===References===

Latest revision as of 14:24, 5 October 2011

RBS+ split sfGFP 11 + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A RBS and a start codon were added to the front of the CDS of sfGFP11 to initiate translation of sfGFP11.

Source

Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908

References

Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107

Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854

Jun Zhou, Jian Lin, Cuihong Zhou, Xiaoyan Deng and Bin Xia.(2011).An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein.Acta Biochim Biophys Sin 43 (3): 239-244.