Difference between revisions of "Part:BBa K524007"
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<partinfo>BBa_K524007 short</partinfo> | <partinfo>BBa_K524007 short</partinfo> | ||
| − | The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence. | + | The split sfGFP1-10 was constructed from an pBAD driven sfGFP [https://parts.igem.org/Part:BBa_I746908 BBa_I746908]. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:23, 5 October 2011
RBS+ split sfGFP 11 + double terminator
The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
Usage and Biology
When driven by an appropriate promoter, the product of this biobrick should complement with the product of biobrick sfGFP11 BBa_K524006 to give green fluorescence under UV.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]