Difference between revisions of "Part:BBa K524002"
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<partinfo>BBa_K524002 short</partinfo> | <partinfo>BBa_K524002 short</partinfo> | ||
| − | The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 215-238 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence. | + | The split sfGFP1-10 was constructed from an pBAD driven sfGFP [https://parts.igem.org/Part:BBa_I746908 BBa_I746908]. CDS of sfGFP includes amino acid residues 215-238 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:22, 5 October 2011
pLac + RBS+ split sfGFP11 + double terminator
The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 215-238 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
Usage and Biology
The split sfGFP1-10 in this biobrick is assembled behind R0010 Lac promoter.
Induced by IPTG, the product of this biobrick should complement with the product of IPTG inducible biobrick sfGFP1-10 BBa_K524001 to give green fluorescence under UV.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]