Difference between revisions of "Part:BBa J23103:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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<partinfo>BBa_J23103 AddReview 5</partinfo>
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<I>iGEM HKU 2011</I>
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To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain.
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The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression.
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|[[Image:MRFP data graph.png|450px|thumb|center|mRFP fluorescence intensity under different promoters]]||
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Revision as of 14:15, 5 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J23103

User Reviews

UNIQ6166256c7a25afdc-partinfo-00000000-QINU

•••••

iGEM HKU 2011

To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain. The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression.

mRFP fluorescence intensity under different promoters

UNIQ6166256c7a25afdc-partinfo-00000002-QINU