Difference between revisions of "Part:BBa K598014"
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<partinfo>BBa_K598014 short</partinfo> | <partinfo>BBa_K598014 short</partinfo> | ||
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This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron. | This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron. | ||
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+ | It is the positive control of the theophylline inducing experiment. For further information, please see ,[https://parts.igem.org/Part:BBa_K598013#Reference BBa_K598013]. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:08, 5 October 2011
pBAD+HH+CdIntron+E0040+b0015
This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
It is the positive control of the theophylline inducing experiment. For further information, please see ,BBa_K598013.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1408