Difference between revisions of "Part:BBa K624001"
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| − | + | These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left, above graph is control group of E.coli strain DH5a. The right, above is tetR+RBS+GFP in plasmid c3 in E.coli , while the below is plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing is under operation. | |
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Revision as of 13:59, 5 October 2011
tetR+RBS+GFP+ter
Add a promoter and a RBS on the upstream will express tetR protein and GFP.
GFP can help detecting tetR expression.
These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left, above graph is control group of E.coli strain DH5a. The right, above is tetR+RBS+GFP in plasmid c3 in E.coli , while the below is plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing is under operation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1358