Difference between revisions of "Part:BBa K658017:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K658017=== | ===Applications of BBa_K658017=== | ||
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| + | The lux pR strength testing device BBa_K658017 with other lux pR strength testing devices BBa_K658016 BBa_K658018 BBa_K658019 was first cloned into plasmid pSB1A2 respectively, followed by transformation into E.coli strain BL21. Fluorescence was measured when cell growth reached a steady state (around 20h). | ||
| + | The results are shown in following figures: | ||
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| + | [[Image:XMU_China_4beta.jpg|left|Figure 4 Promoters strength relative to lux pR-3 (BBa_K658006)|frame|Figure 4 Promoters strength relative to lux pR-3 (BBa_K658006).]] | ||
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| + | [[Image:XMU_China_5beta.jpg|left|Figure 5 Efficiency of promoter lux pR (BBa_R0062) and its three mutants | ||
| + | |frame|Figure 5 Efficiency of promoter lux pR (BBa_R0062) and its three mutants.]] | ||
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| + | [[Image:XMU_China_6beta.jpg|left|Figure 6: Fluorescence of four IR-GFP devices at 20h. 5, 3, 3/5, N represent for IR-3-GFP (BBa_K658017), IR-5-GFP (BBa_K658018), IR-3/5-GFP (BBa_K658019) and IR-GFP (BBa_K658016) respectively|frame|Figure 6: Fluorescence of four IR-GFP devices at 20h. 5, 3, 3/5, N represent for IR-3-GFP (BBa_K658017), IR-5-GFP (BBa_K658018), IR-3/5-GFP (BBa_K658019) and IR-GFP (BBa_K658016) respectively.]] | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg"> | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 13:58, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K658017
The lux pR strength testing device BBa_K658017 with other lux pR strength testing devices BBa_K658016 BBa_K658018 BBa_K658019 was first cloned into plasmid pSB1A2 respectively, followed by transformation into E.coli strain BL21. Fluorescence was measured when cell growth reached a steady state (around 20h). The results are shown in following figures:
Figure 4 Promoters strength relative to lux pR-3 (BBa_K658006).
Figure 5 Efficiency of promoter lux pR (BBa_R0062) and its three mutants.
Figure 6: Fluorescence of four IR-GFP devices at 20h. 5, 3, 3/5, N represent for IR-3-GFP (BBa_K658017), IR-5-GFP (BBa_K658018), IR-3/5-GFP (BBa_K658019) and IR-GFP (BBa_K658016) respectively.
User Reviews
UNIQ8c7829149e664dac-partinfo-00000001-QINU UNIQ8c7829149e664dac-partinfo-00000002-QINU