Difference between revisions of "Part:BBa K533001:Experience"

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Latest revision as of 13:32, 5 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K533001

When this fusion protein is expressed in E. coli, it will enable specific binding to proteins with a multi-proline peptide.

We tested interaction between OmpA-HIV protease site-SH3 and multi-proline mCherry. The result is also applicable to this part with OmpA and SH3 only.

E. coli with the plasmid for OmpA-HIV site-SH3 is induced with 0.5mM IPTG at 18 centigrade for 12 hours before harvesting by mild centrifuge.

E. coli expressing OmpA-HIV protease site-SH3 was mixed with mCherry solution at room temperature for 30 minutes.

After washing with distilled water twice, some of the bacteria were fixed on a slip and stained with DAPI.

Color Channel E. coli with IPTG induction E. coli without induction
mCherry Thuexp bind p.png Thuexp bind n.png
DAPI Thuexp bind p d.png Thuexp bind n d.png
merge Thuexp bind p m.png Thuexp bind n m.png

User Reviews

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