Difference between revisions of "Part:BBa K658004"

Latest revision as of 12:37, 5 October 2011

a bacteria population-control device with RBS0.3 driven by lacl+pL

The device is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri.It is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 718
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2345
Illegal BsaI.rc site found at 1874

Revision as of 11:31, 5 October 2011 (view source) Xmuwendy (Talk \| contribs) ← Older edit		Latest revision as of 12:37, 5 October 2011 (view source) Xmuwendy (Talk \| contribs)
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	<partinfo>BBa_K658004 short</partinfo>		<partinfo>BBa_K658004 short</partinfo>

−	The device is ~~constructed~~ based on the ~~Biobrick part BBa_K658003~~. ~~This device~~ is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer~~. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri~~. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.	+	The device is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri.It is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.